Intermediate

Part:BBa_S04317

Designed by: Chie Umatani(team Todai-Tokyo member)   Group: Guests   (2009-10-18)

J63006:K179001

yeast constitutive Gal1 promoter and leucine dehydrogenase[http://www.uniprot.org/uniprot/?query=YqiT&sort=score]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 977


Functional Parameters

We measured the intensity of smell of cleansed air, air, air in the test tube in which we were shaking culture of E.coli “negative control”, and air in the test tube in which we were shaking culture of E.coli which has plasmid containing “promoter + RBS + yqiT + double terminator”

Smell.png

Data
Time for shake culture (each of sample) 15 hours
Negative control has plasmid containing “yqiT + double terminator” (Not including promoter)
Cleansed air is cleansed with active carbon filter.
Equipment

  • Trade name: ODOR CONCENTRATION METER
  • Model: XP-329
  • Serial No. 311059
  • Manuf. NEW COSMOS ELECTRIC CO,LTD.
  • Setting: Peak hold


[edit]
Categories
Parameters
None